Influence of two kinds of PARP-1 inhibitor on the proliferation and apoptosis of ECA-109 cells induced by hydroxy camptothecin

Objective To explore the effects of two kinds of PARP-1 inhibitors NU1025 and AG14361 on the proliferation and apoptosis of ECA-109 cells induced by the anti-cancer drug hydroxy camptothecin.Methods The ECA-109 cells were routinely cultured and treated with HCPT,HCPT +NU1025 and HCPT+AG14361 for 24 h.Then MTT method was employed to evaluate the proliferation of ECA-109 cells.Flow cytometer annexin V-FITC/PI double staining method was enrolled to analyze the apoptosis the ECA-109 cells,and comet assay was employed to assess the DNA damage content of the ECA109 cells.Results Compared with the control and HCPT treated groups,HCPT+NU1025 and HCPT+AG14361 treatment significantly reduced cell proliferation,increased cell apoptosis and aggravated cell DNA damage(P 0.05).Conclusion The PARP-1 inhibitor NU1025 and AG14361 both can increase sensitivity of ECA-109 cells in response to HCPT through inhibiting PARP-1 activity,blocked DNA damage and repair.NU1025 has advantage of lower cytotoxicity and higher sensitizing activities and can provide a new target for the clinical treatment of esophageal cancer.