The method of manufacturing a color filter substrate, the color filter substrate and a display device

The present invention provides a method of manufacturing a color filter substrate, the color filter substrate and a display device, the method comprising: providing a substrate; forming a color pixel unit on the substrate; forming a protective layer on the color pixel unit; in sequentially applying on the protective layer coating composition to the mat with membrane dope and septum, and once or twice by a patterning process to form alignment layers and spacers. The technical solution of the present invention, it is possible to save production line color filter substrate transport time, reduce the production process, to improve the production cycle, reduce production costs.