Anti-diabetic effect of the ethyl acetate fraction of Clerodendrum volubile: protocatechuic acid suppresses phagocytic oxidative burst and modulates inflammatory cytokines

Abstract The antidiabetic effects of the ethyl acetate (EtOAc) fraction of Clerodendrum volubile leaves was investigated in this study. EtOAc extract was also fractionated to isolate the active compounds. The structure of the isolated compound (Protocatechuic acid) was established using 1 H and 13 C NMR spectroscopies and mass spectrometry. Protocatechuic acid was investigated for its anti-oxidative burst in polymorphonuclear neutrophils (PMNs) and macrophages. It was also docked with α–glucosidase and TNF-α. Acute treatment with EtOAc fraction of Clerodendrum volubile leaves significantly (p  C. volubile on type 2 diabetes and its complications, which can be attributed to the main bioactive compound, protocatechuic acid.