DNA probes to repetitive sequences for the analysis of porcine genomic DNA with reference to DNA methylation.

Abstract The aim of this study was to isolate probes to repetitive sequences of porcine (Great Yorkshire × Landrace) genomic DNA. The production of transgenic animals involves the isolation of stem cell lines and the understanding of DNA methylation modifications. Probes to repetitive sequences enable the analysis of DNA methylation in the tissues of various embryonic stages of the pig. A primary library of porcine genomic DNA was screened with labeled fragments of porcine DNA, and 6 clones containing repetitive DNA were isolated and analyzed for the presence of potential methylation moieties (CCGG sites). Probes of all 6 clones were tested in a hybridization analysis of HpaII and MspI digests of porcine sperm DNA, and it was found that methylation was not present in the methylation moieties of the repetitive sequences.