Immunoblotting reagent kit of syphilis specific total antibodies and preparing method thereof

The invention provides an immunoblotting reagent kit of syphilis specific total antibodies and preparing method thereof, relating to a reagent kit. The reagent kit is provided with a carrier plate, a nitrocellulose membrane, a syphilis specific total antibody testing line and a control line, wherein the syphilis specific total antibody testing line and the control line are orderly arranged on the nitrocellulose membrane; the syphilis specific total antibody testing line is coated with syphilis specific recombinant antigens; the control line is coated with a human total antibody; and horseradish peroxidase is marked with an anti-human Ig antibody. The method comprises the following steps of: firstly preparing the syphilis specific recombinant antigens; applying samples of the nitrocellulose membrane; preparing the anti-human Ig monoclonal antibody; marking the anti-human Ig monoclonal antibody on the horseradish peroxidase; and preparing the immunoblotting reagent kit. The method can be used for testing the syphilis specific total antibodies in the specimens of whole blood, serum, blood plasma, cerebrospinal fluid and the like. The invention has the following advantages: during testing, the needed specimen quantity is small, special instruments are not needed, testing is simple, convenient and rapid; the specificity is strong, the sensitivity is high, testing is accurate and reliable, the cost is low and the invention is widely applied.