Fingerprint analysis of Luotong capsule by HPLC

Objective: To develop the chromatographic fingerprints of Luotong capsule by HPLC.Methods: The separation was performed on YMC-PacK ODS(4.6 mm×250 mm, 5 μm)column with a mobile phase consisting of acetonitrile-0.5% phosphoric aicd as gradient elution at the flow rate of 1.0 ml/min.The detective wavelength was 276 nm.The column temperature was 30℃.Results: A standard HPLC fingerprint procedure was developed for Luotong capsule with 18 common peaks.The similarity of 10 batches of Luotong capsule was not lower than 0.978.Conclusion: The mehtod is accurate,repeatable and useful for the quality control of Luotong capsule.