Raman spectroscopy for monitoring of organic and mineral structure of bone grafts

The results of experimental studies of donor bone samples (rat, rabbit and human) with varying degrees of mineralisation by Raman spectroscopy were presented. Raman spectra were obtained for the Raman bands 950-962 (РО 4 ) 3- , 1065-1070 (СО 3 ) 2- and 1665 cm-1 (Amide I). In demineralized bone a sharp decline (to 98 %) in the range of 950-962 cm-1 (РО 4 ) 3- and 1065 - 1070 cm -1 was observed. This decrease was accompanied by the emergence of the 1079-1090 cm-1 band corresponding to the hydrated state СО 3 2- .