Purification and properties of alpha-galactosidase isosymes from Phlebia radiata.

Phlebia radiata formed extracellular α-galactosidase when it was grown in a culture containing wheat bran or locus bean gum as a carbon source. Their activities were optimal at pH 5.0, and demonstrated the highest level of activity at 60°C. Highly purified isoforms of α-galactosidase (AGaS-ml, AGaS-m2, AGaS-m3) isolated from the media with galactomannan and (AGaS-bl, AGaS-b2, AGaS-b3) from the media with wheat bran were obtained by means of the column chromatography on Q-Sepharose and chromatofosussing on Polybuffer Exchanger PBE-94.