Purification procedures for synthetic dyes: Part 1—dry column chromatography

Abstract The suitability of dry column chromatography for the purification of synthetic dyestuffs has been investigated. The results of this study indicate that the utility of this method for the generation of gram quantities of pure dyestuffs is limited, from a practical standpoint, to disperse dyes. Although dry column chromatography appears to be impractical for the purification of gram quantities of acid and direct dyes, relatively simple monoazo acid dyes were successfully purified in smaller quantities by this procedure. The simplicity and low cost of this method make it an excellent way to obtain useful amounts of disperse dyes which are pure enough for use in biological assays such as Ames tests.