Nanofibrous scaffolds of epsilon-polycaprolactone containing Sr/Se-hydroxyapatite/ graphene oxide for tissue engineering applications.

For wound healing applications, a scaffold of biocompatible/porous network is crucial to support cell proliferation and spreading. Therefore, -polycaprolactone (PCL) nanofibrous scaffold containing co-dopants of strontium/selenium into hydroxyapatite (HAP) were modified with different contributions of graphene oxide (GO) via the laser ablation technique. The obtained compositions were investigated using XRD, TEM and FESEM. It was obvious that fiber diameters were in the range of from 0.15- 0.30 microm and 0.35-0.83 microm at the lowest and highest concentration of GO respectively, while the maximum height of the roughness progressed to be 393 nm. The toughness behavior is promoted from 5.77+/-0.21 to be 9.16+/-0.29 MJ/m3 upon GO from lowest to the highest contribution, while the maximum strain at break reached to 148.1+/-0.49% at the highest concentration of GO. The cell viability indicated that the fibrous scaffold was biocompatible. The investigated of HFB4 cell attachments towards the fibrous compositions showed that with the rising of GO, cells tend to grow intensively through the scaffolds. Furthermore, the proliferation of cells was observed to be rooted in the porous structure and spreading on the surface of the scaffold. This progression of cells with increasing of GO content may provide a simple strategy not only to enhance mechanical properties but also to manipulate a nanofibrous scaffold with proper behaviors for biomedical applications.