Effect of Combination Yeast Bread and Chlorella sp. to Population Growth and Protein Content of Brachionus plicatilis

One of larvae feed can be used is Brachionus plicatilis but necessary an enrichment for review adding nutritional values of the Brachionus plicatilis . Some phytoplankton frequently used type food by Brachionus plicatilis between lay Tetraselmis sp., Skeletonema costatum , Chlorella sp., Dunaliella salina and Spirulina sp. (Isnansetyo And Kurniastuty, 1995). In this time, many parlors seed microalgae replace with yeast bread, beside that yeast bread serves as a probiotic too (Winasuria, 1993). This study aims to determine the effect feeding review form a combination of yeast bread and Chlorella sp. against population growth and protein content of Brachionus plicatilis . The research method used is an experimental method with completely randomized design as design of Experiments. The treatment used is a combination of yeast and Chlorella sp. with different period, that was treatment A ( Chlorella sp. 100%), treatment B ( Chlorella sp. 25% and 75% baker's yeast), treatment C ( Chlorella sp. 50% and 50 % of baker's yeast), treatment D ( Chlorella sp. 75% and 25% baker's yeast), each treatment was repeated 5 times. The main parameter observed is population growth, growth rate and protein content. The supporting parameters observed parameter is water quality. Data analysis using variant analysis (ANOVA) and to review the best treatment, Duncan's Multiple Range Test performed. Results Provision combination of baker's yeast and Chlorella sp. with term certain showed significant differences (p<0,05) against population growth Brachionus plicatilis . The highest population growth are in treatment D  and the lowest are in treatment B. For a review feeding of Brachionus plicatilis preferably using Chlorella sp. with 1,125x106 density cells / ml and 0,0005 grams of baker's yeast.