Treatment of honghua injection acupoint iontophoresis on traumatic vitreous hemorrhage

Objective To investigate the effect and pharmcokinetic role of honghua injection acupoint iontophoresis on traumatic vitreous hemorrhage.Methods The traumatic vitreous hemorrhage model was induced by injecting into vitreous of 0.2 mL autohemorrhage in 36 eyes of 18 New Zealand white rabbits.2 ml of honghua solution was iontophorezed via acupoint iontophoresis in the Qingming,Chengqi,Cuanzhu,Laogong for 15 minutes per time and twice per day for 10 times in honghua-treated group (9 rabbits),and 9 model rabbits were as control group.Aqueous humor and vitreous were withdrawn at different time,and the drug concentration in aqueous humor and vitreous were detected with high-pressure liquid chromatography (HPLC).The absorption status of vitreous hemorrhage was clinically evaluated by ophthalmoscope.Results The aqueous humor regression equation of honghua peak area (Y) and honghua concentration (X) was Y=32.486 X-5.154,presenting a good linear correlation between them (r =0.9989,P ＜0.01).The vitreous regression equation of honghua peak area (Y) and honghua concentration (X) was Y=30.462 X-5.024,presenting a good linear correlation between them (r =0.9951,P ＜0.01).There was no significant difference in honghua concentration between vitreous and aqueous humor in 0.5 hours and 2 hours (P ＞0.05),but significant difference was found in 5 hours (P ＜0.01).No obvious difference was seen in visuality of fundus before treatment between the two groups.In honghua-treated group,the absorption of vitreous hemorrhage was quicker than in the control group,and the number of visual fundus was remarkably increased in comparison with control group,showing that the effect of honghua injection-iontophoresis was better than that in control group.Conclusions For traumatic vitreous hemorrhage,honghua injection-iontophoresis is an effective and simple approach to accelerating the absorption of vitreous hemorrhage and shorten the course of treatment. Key words: Acupoint iontophoresis;  Vitreous hemorrhage;  Honghua