Preparation method for polymethyl methacrylate microsphere immobilized luciferase

The invention belongs to the field of enzyme engineering and particularly relates to a preparation method for polymethyl methacrylate microsphere immobilized luciferase. The method comprises the steps of magnetic microsphere preparation and luciferase immobilization. Through treatment by the method, the luciferase properties are very stable, the efficiency is improved, the catalytic reaction process is easy to control, the mechanical strength is high, and the luciferase is reusable and very suitable for application to the high-flux genome sequencing process.