The effect of exogenous PDGF-DD protein on human hepatic carcinoma cell line BEL-7402 in vitro

Objective To investigate the effect of exogenous PDGF-DD protein on proliferation and metastasis related gene(VEGF and CXCR4) of PDGF-D and β-PDGFR positive expressed human hepatic carcinoma cell line BEL-7402.Methods BEL-7402 cells were exposed to different concentrations of PDGF-DD protein(0,5,10,20,50,100 pg/mL) for 48 h,and cell proliferation was detected by MTT assay.The flow cytometer (FCM) was used to analyze the distribution of cells cycle.The mRNA expressions of VEGF and CXCR4 were detected by RT-PCR.Results Exogenous PDGF-DD protein promoted proliferation of cells in a dose-dependent manner from 10 pg/mL to 100 pg/mL(P 0.05).Incubation of tumor cells with PDGF-DD markedly decreased the percentage of the G0/G1 -phase and increased that of the S-phase.Compared with control group (0 pg/mL),mRNA expression of VEGF and CXCR4 (from 10 pg/mL to 100 pg/mL) were increased(P 0.05).Conclusions Exogenous PDGF-D signaling may play a crucial role in the carcinogenesis,development and metastasis of hepatocellular carcinoma.It can be used as a prognostic factor and a potential target for therapy in hepatocellular carcinoma.