In vitro spectroscopic study of normal and pathological blood denaturation

By means of a systematic spectroscopic study of an in vitro blood clot model, iron compounds involved in blood denaturation have been identified and evaluated at each stage of blood denaturation. So, a blood denaturation pattern is available for normal blood, but also for some pathological bloods (polyglobulic, Β-thalassemic and drepanocytic). Normal blood denaturation stands for a reference that will be used for several purposes. Thus, this study compares normal and pathological blood denaturation rates. Polyglobulic and Β-thalassemic blood denaturations are quite similar to normal blood denaturation. On the opposite, drepanocytic blood denaturation is really different (some hemochromes are not observed) and is a lot slower. This delay can be attributed to deoxyhemoglobin S fiber formation that could protect trapped compounds from oxidization.