Metapontum Forest Reserve: Salt Stress Responses in Pinus halepensis
2013
Metapontum Forest
Reserve is an artificial formation located between mouths of Bradano and Basento
river, it is composed prevalently of Aleppo pine (Pinus halepensis). In recent
years, the Metapontum
coast is characterized by a strong erosive process which has really removed the
dune behind the beach moving in the inland and causing the decline of the historical
pinewood. This negative effect on plant was induced by an increase in soil salinity,
which is one of the major abiotic stresses. A clear understanding of the molecular
mechanisms involved in plants response to salt stress is fundamentally important
for plant biology. The salinity soil causes broad variety of physiological and biochemical
processes, as oxidative damage, also has a negative effect on energy metabolism,
which unavoidably resulted in a decreased ATP production through photophosphorylation
and, thus, affected the Calvin cycle in photosynthesis. A proteomic approach was utilized
to identify key protein which result to be directly responsive to salt stress. Total
proteins were extracted from the leaves by a combination of TCA—acetone and phenol, and separated by two-dimensional gel electrophoresis
at pH 5 - 8. Spots were stained with Coomassie Brilliant Blue and analyzed with the
software PDQuest 8.0 (Bio-Rad) to identify differentially expressed polypeptides.
Preliminary analysis revealed around 29 differentially expressed proteins, and they
were sequenced by MALDI TOF and LC-MS/MS. Sequenced spots were classified in different
functional classes.
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