LncRNA RMST Suppressed GBM Cells Mitophagy through Enhancing FUS SUMOylation

Abstract Background Long non-coding RNAs (lncRNAs) play significant role in post-translational modifications of proteins, yet the importance of lncRNAs for sumoylation is unknown. Methods RMST expression in glioma tissues and normal brain tissues was measured by RT-qPCR and in situ hybridization.The functional roles of RMST in astrocytomas were demonstrated by a series of in vitro experiments. The potentialmechanisms of RMST for sumoylation was investigatedby RNA immunoprecipitation,RNA pull-down,western blotting and co-immunoprecipitation assays. Findings We first demonstrated the oncogenic activity of lncRNA RMST by inhibiting glioma cells mitophagy. We also first determined that RMST is an enhancer of FUS SUMOylation, especially boosting SUMO1 modification at K333. SUMOylation induced by RMST contributes to the interaction between FUS and hnRNPD and stabilized their expression and cells mitophagy. Importantly, LncRNA RMST could serve as a promising prognostic factor for glioma patients. Interpretation our results demonstrated a previously unknown function of lncRNAs worked as an enhancer in FUS SUMOylation, and RMST will be a significant guide for the development of medications targeting gliomas.