[Multistaged cryopreservation of tympano-ossicular grafts. I. Preservation procedures].

: Good conservation of tympano-ossicular grafts is still actual. Theoretical principles of cryobiological action and the detailed description of applied method are given. Newly developed multistaged cryoprocedure in liquid nitrogen AT-196 degrees C with 9% DMSO showed well the microscopic appearance of connective tissue transplants without alterations of metabolical and mineral content. The conservation was mainly successful in peripheral, perivascular and perihaversian regions, while central zones gave signs of hypothermic lesion.