Response surface optimization of medium composition for xylitol production by Debaryomyces hansenii var hansenii using corncob hemicellulose hydrolysate
2013
Optimization of the culture medium for xylitol production using Debrayomyces
hansenii var hansenii was carried out. The optimization of xylitol production
using corncob hemicelluloses hydrolysate as substrate was performed with
statistical methodology based on experimental designs. The screening of nine
nutrients for their influence of xylitol production to achieved using a
Plackett-Burman design. MgSO4.7H2O, KH2PO4, (NH4)2SO4, yeast extract were
selected for based on their positive influence on xylitol production. The
selected components were optimized using Response Surface Methodology (RSM).
The optimum conditions are: MgSO4.7H2O - 1.02 g/l, (NH4)2SO4 - 3.94 g/l,
KH2PO4- 2.74 g/l and yeast extract - 3.45 g/l. These conditions are validated
experimentally which revealed an enhanced xylitol yield of 0.76 g/g.
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