The effect of inhaled nitric oxide on the carrageenan-induced paw edema
2015
Inhaled nitric oxide therapy reaches not only
pulmonary vessels, but also other vasculatures,
presenting anti-inflammatory effects. Therefore, this
study investigated the effects of inhaled nitric oxide on a
mice model of carrageenan-induced paw edema. Paw
edema was induced in male Swiss mice (20-30 g) by
subplantar injection of carrageenan (0.05 ml of a 1%
suspension in 0.9% saline). The evaluation of timecourse
edema (mililiter) was measured by
plethysmometry until 12 h following carrageenan
administration. Thirty minutes after carrageenan
injection, some groups received inhaled nitric oxide (300
ppm at variable doses and times) or Indometacin (INDO
5 mg/Kg, v.o), while others received sildenafil (1
mg/Kg, i.p) or rolipram (3 mg/Kg, i.p.) with or without
inhaled nitric oxide. Paws were assessed for edema
levels by plethysmometry, mieloperoxidase activity and
histological analysis. Inhaled nitric oxide significantly
reduced carrageenan-induced paw edema,
mieloperoxidase activity and inflammatory infiltrate,
although similar results were also observed in sildenafil
and rolipram treated groups. In addition, significant
effects between inhaled nitric oxide with pharmacological
therapy was observed. Inhaled nitric oxide
presents anti-inflammatory effects on carrageenaninduce
paw edema, as observed through reduced edema,
mieloperoxidase activity and neutrophil infiltration,
indicating that inhaled nitric oxide therapy goes beyond
lung vascular effects.
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