机械压应力作用下IL-1α与TNF-α对滑膜细胞MMP-2，-9活性的影响

Objective To explore the role of synovium in the process of anterior cruciate ligament (ACL) injury by studying the effects of inflammatory on the activities of MMP-2,-9 under mechanical compression in human synoviocytes. Method We used an equi-biaxial stretch chamber with 12% mechanical injury compression and inflammatory factors (IL-1α and TNF-α) in human synoviocytes, and detected the activities of MMP-2,-9 under different conditions byzymography. Result Mechanical compression increased the MMP-2 production by 122% under 12% compression. In addition, TNF-αcan also elevate the actMtyofMMP-2 in a time dependent way, while IL-α cannot. However, mixture of these two factors dramatically increased MMP-2,-9 production. Mechanical injury had a strong synergistic effect on MMP-2 -9 productions with mw-a and IL-1α. Conclusions Mechanical and inflammatory factors can elevate the activeity of MMP-2,-9. Thus, synvvium might involve in regulating the micro-environment of joint cavity and the injury/healing process of knee joint tissue.