Preparation method of human serum albumin gold nanoclusters

The invention belongs to the technical field of nano materials, and specifically relates to a preparation method of human serum albumin gold nanoclusters. The method comprises the steps of 1) slowly adding a chloroauric acid solution to a human serum albumin solution, highly agitating, then adding a NaOH solution to regulate the pH value to be 11 to 12.5, and ultrasonically processing the mixed solution according to selected ultrasonic opening-closing ratio under the room temperature of 37 DEG C so as to obtain a gold nanoclusters solution; 2) performing conjugating precipitation for the gold nanoclusters solution and an ZnCl2 solution, centrifuging, and washing the precipitate through distilled water, dissolving the precipitate into a PBS buffer solution again, respectively dialyzing in the PBS buffer solution and deionized water to obtain a human serum albumin gold nanoclusters solution. According to the method, human serum albumin is used to replace heterologous protein to be used as a protecting agent and a reducing agent to prepare the gold nanoclusters, so that the immunogenicity of a human body can be removed; gold nanoclusters being small in particle size, uniform in distribution, high in quantum output and high in near infrared fluorescence can be prepared within a short time.