Fabrication of Gehlenite Nanopowder Containing Electrospun Nanofibers for Bone Tissue Engineering

Bioactive Polycaprolactone/Collagen nanofibers containing gehlenite Nano-powder were fabricated using electrospinning for bone tissue engineering. The physicochemical properties of scaffolds measured using scanning electron microscopy, tensile test, FTIR spectroscopy, and water contact angle measurement test. A slight enhancement (∼20 nm) in fibers diameter was observed by adding gehlenite nanoparticles to the scaffolds, but the fiber diameter distributions for both scaffolds were within the normal range of extracellular matrix (50–500 nm). In vitro experiments demonstrated well attachment and higher proliferation of MG-63 cells on gehlenite nanoparticles containing scaffolds. Besides, the stimulatory cell differentiation effects of Polycaprolactone/Collagen/gehlenite scaffolds were demonstrated by Alizarin red staining. This study represents for advanced of the natural and synthetic polymers for bone tissue engineering.