Cloning and sequence analysis of Fas gene of pig

The Fas gene was amplified from the total RNA from PK-15 cells using reverse transcription polymerase chain reaction(RT-PCR) and cloned into PMD18T vector,and then sequenced.The sequence analysis of Fas gene showed that 100% shares homology with that of published in GenBank.The nucleotide and the deduced amino acid sequences of the Fas gene of pig shared 73.4%,79.2%,76.4% and 56.2%,67.0%,64.6% with those of human,bovine and sheep,respectively.The intracellular portion of Fas containing death domain was hight homology among of pig,human,bovine and sheep.