Effect of incubation temperature on the creatine kinase release from an in vitro rat skeletal muscle preparation

Abstract 1. 1. The effect of hyperthermia on muscle release of creatine kinase (CK) was studied on an in vitro rat muscle (extensor digitorum longus) preparation. 2. 2. Release of CK from muscle was more important at 41°C than at 36°C during an in vitro test. 3. 3. After a steady-state phase, an increase in the muscle CK release appeared, before any mechanic index of muscle viability alteration. 4. 4. This final increase of CK release was observed at 36°C and at 41°C, and it could be energy dependent.