tHcy Detected by High Performance Liquid Chromatography will be a Dangerous Factor for the Invasion of Occlusive Vascular Disease

Objective:To study the clinical application of high performance liquid chromatography（HPLC)、Radioimmunodetection and Automatic biochemical Analyzer on the detection of plasma total homocysteine(tHcy)、folic acid（FA）、VitB12、Triglyceride（TG）、total cholesterol(Ch)、high density lipoprotein cholesferol(HDL-C) and low density lipoprotein cholestorol(LDL-C).To manifest mechanism of the increasing of tHcy leading to diabetic retinopathy.Methods:The levels of plasma tHcy 、folic acid、VitB12、blood sugar、 blood pressure and blood lipid were detected by the instrumentations above in diabetic retinopathy cases.Results:The levels of plasma tHcy in diabetic retinopathy group were much higher than that in the control group.Conclusions:We discovered biomarkers of diabetic retinopathy.HPLC method can be used well for the detection of tHcy.