REPONSE A LA CULTURE IN VITRO DE TROIS VARIETES DE L'OLIVIER

0.5 mg.l -1 de Kinetine + 0.5 mg.l -1 d'ANA et sur milieu B5 additionne de 0.4 mg.l -1 de BAP + 1.33 mg.l -1 d'AIB, et chez Siguoise sur milieu (MS/2) additionne de 0.5 mg.l -1 de Kinitine + 1 mg.l -1 d'ANA. Les pousses feuillees enracinees de ces deux varietes ont subi la phase d'acclimatation avec succes et se sont adaptees a la croissance sous serre. Mots cles - Olea europaea L. - culture in vitro - milieu de base - regulateurs de croissance. Abstract The experiment conducted in the Laboratory of Genetic, Biochemistry and plant Biotechnology (LGBBV), Faculty of Sciences, Mentouri University (Constantine) aimed to study the response of three olive cultivars (Olea europaea L.) response to two basic tissue culture media complemented with different concentrations of growth regulators. Cuttings of Chemlal, Siguoise and Grosse de Hamma cultivars were collected from the Erkani Pilot Farm located at Hamma Bouziane. Under complete aseptic conditions explants 1cm long holding lateral buds were subcultured in MS and B5 media complemented with growth regulators. B5 medium emerged as the best for tissue culture of the studied cultivars. Based on leaf proliferation, stem extension and accumulated fresh matter measurements, Chemlal showed a good response to in vitro culture. Root proliferation was induced after callus formation for Chemlal in the 1/2 diluted MS medium complemented with 0.5 mg.l -1 Kinetine + 0.5 mg.l -1 ANA and in B5 medium complemented with 0.4 mg.l -1 BAP + 1.33 mg.l -1 AIB. And for Sigoise in the 1/2 diluted MS medium complemented with 0.5mg.l -1 Kinetine + 1 mg.l -1 ANA. Leafed and rooted explants from the two cultivars have passed successfully the acclimation phase and fully adapted to grow under glasshouse conditions.