Highly efficient Cas9-mediated transcriptional programming

Alejandro Chavez,Jonathan Scheiman,Suhani Vora,Benjamin W. Pruitt,Marcelle Tuttle,Eswar Prasad R. Iyer,Shuailiang Lin,Samira Kiani,Christopher D. Guzman,Daniel J. Wiegand,Dmitry Ter-Ovanesyan,Jonathan L. Braff,Noah Davidsohn,Benjamin E. Housden,Norbert Perrimon,Ron Weiss,John Aach,James J. Collins,George M. Church

Highly efficient Cas9-mediated transcriptional programming

2015

Alejandro Chavez
Jonathan Scheiman
Suhani Vora
Benjamin W. Pruitt
Marcelle Tuttle
Eswar Prasad R. Iyer
Shuailiang Lin
Samira Kiani
Christopher D. Guzman
Daniel J. Wiegand
Dmitry Ter-Ovanesyan
Jonathan L. Braff
Noah Davidsohn
Benjamin E. Housden
Norbert Perrimon
Ron Weiss
John Aach
James J. Collins
George M. Church

The fusion of three transcriptional activation domains to a nuclease-deficient Cas9 achieves robust induction of gene expression and can induce differentiation of hiPSCs.

Keywords:

Gene expression
Induced pluripotent stem cell
Gene targeting
Regulation of gene expression
Molecular biology
Cas9
Cellular differentiation
Bioinformatics
HEK 293 cells
Synthetic biology
Biology
Epigenome editing

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