Effect of carbachol on gut barrier function in resuscitation of burn shock with oral rehydration

Objective To investigate the effect of carbachol on gut barrier function in the resuscitation of burn shock with oral rehydration in dog.Methods 20 adult male Beagle dogs with 35% TBSAⅢ° burn by napalm combustion method were randomly divided into delayed resuscitation (DR) group, peroral glucose electrolyte solution (GES) group,peroral carbachol (CAL) group, and peroral GES plus carbachol(GES+CAL) group. According to Parkland formula(4 mL· kg-1·1%TBSA-1), GES was given oral by a gastric tube. Carbachol (20 μg/kg) was dissolved normal sodium and took orally by a gastric tube at 30min and 4 h post burn. Four groups likewise treat with delayed venous-fluid resuscitation with 5% glucose in normal saline after 24 h post burn. blood diamine oxidase (DAO),D-lactic acid (D-LA) and D-xylems(D-XY) of each animal were detected at preinjury and 2,4,8,24,48,72 hours post burn.Result Levels of blood DAO,D-LA and D-XY of DR group were significantly higher comparing with other three groups(P0.01 P0.05). Group comparison of each treatment,blood DAO in GES+CAL group was lower than GES group and CAL group in 2 h and 4 h post burn. In 8 h and 24 h post burn, level of blood D-XY in GES/CAL group was lower than GES group and CAL group(P0.05).Conclusion It is indicated that carbachol have effect on protecting gut barrier function in the resuscitation of burn shock with oral rehydration in dog.