A Microdialysis Workflow for Metabotyping Superficial Pathologies: Application to Burn Injury

Burn injury can be a devastating traumatic injury, with long-term personal and social implications for the patient. The many complex local and disseminating pathological processes underlying burn injury’s clinical challenges are orchestrated from the site of injury and develop over time, yet few studies of the molecular basis of these mechanisms specifically explore the local signalling environment. Those that do are typically destructive in nature and preclude the collection of longitudinal temporal data. Burn injury therefore exemplifies a superficial temporally-dynamic pathology for which experimental sampling typically prioritizes either specificity to the local burn site or continuous collection from circulation. Here, we present an exploratory approach to the targeted elucidation of complex, local, acutely temporally-dynamic interstitia through its application to burn injury. Subcutaneous microdialysis is coupled with ultra performance liquid chromatography – mass spectrometry (UPLC-MS) analysis, pe...