Characterization of Cytochrome f Mutants in the b 6 f - Complex of Chlamydomonas Reinhardtii

Cytochrome f is a chloroplast-encoded protein whose production requires a multi-step maturation process: the petA gene product is converted from a precursor apo-form to a mature holo-form. We used Chlamydomonas reinhardtii whose chloroplast genome can be easily transformed, to produce two site-directed cytochrome f mutants that were expected to be altered in this maturation process: F29L-3 IL and F32P.