Automated enzyme-linked immunosorbent assay using beads in a single tip (BIST) technology coupled with a novel anchor protein for oriented antibody immobilization

An automated enzyme-linked immunosorbent assay (ELISA) system was developed using beads in a single tip (BIST) technology. Sandwich ELISA was performed on glass beads functionalized with a capture antibody via the fusion of antibody-binding streptococcal protein G and a silica/glass-binding protein Si-tag. Our system enables rapid, sensitive detection of antigens.