Implementation of Definite Screening Design in Optimization of In Situ Hydrolysis of EFB in Cholinium Acetate and Locally Produced Cellulase Combined System

Purpose Ionic liquids (ILs) have been found to be highly promising for lignocellulosic biomass pretreatment, due to their excellent abilities to dissolve biopolymers. However, they have found to inactivate enzymes. In previous studies, Cholinium-based ILs showed great compatibility with cellulases and thus were introduced for a combined-system. This study aims to find the optimum condition in order to achieve the maximum pretreatment-hydrolysis of empty fruit bunches (EFB).