Enzyme electrode based on gold-plated polyester cloth

Glucose oxidase is immobilized on gold-plated polyester cloth by entrapment method and coupled with an electron mediator, ferrocene derivative. Cyclic voltammogram depends on the existence of the substrate glucose and the flavo-enzyme was shown to donate electron(s) to the cloth electrode with mediation of the ferrocene derivative even in the immobilized state. The glucose-concentration dependence of the peak current was linear upto several tens of 35 mM, depending on the concentration of the mediator. Co-immobilization of polymer-anchored electron mediator is also studied.