Restriction Endonuclease Chromosome Banding in Tapinoma Nigerrimum (Hymenoptera, Formicidae).

2004 
Fixed metaphase chromosomes and isolated genomic DNA of the ant Tapinoma nigerrimum were treated with the restriction endonucleases EcoRI, Hue111 and Tru91. EcoRI and HaeIII induced a banding pattern similar to G-banding, whereas Tru9I induced a C-band-like pattern. There is only a partial accord between the action of these enzymes on isolated genomic DNA and on DNA in fixed chromosomes. Our results indicate that the presence of target sequences along the chromosomes is necessary but not sufficient to produce chromosome bands. The chromosomal organisation also seems to be important in producing longitudinal differentiation on chromosomes.
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