[Influence of Jiangu decoction on peroxisome proliferator-activated receptor (PPAR) in the femoral head of rabbits with steroid-induced femoral head necrosis].

Objective:The Jiangu decoction(健骨方) is used in the treatment of steroid-induced femoral head necrosis in clinical experiences,which has functions of tonifying kidney and activating blood,and invigorating spleen to remove phlegm.The decoction is mainly composed of Radix Polygoni Multiflori,Rhizoma alismatis Rhizoma Drynariae,haw,medlar,Radix Astragali,radix rehmanniae,angelica,Radix Codonopsis,radix salviae miltiorrhizae,Fructus Ligustri Lucidi,licorice,pharmaceutical composition.This study was designed to investigate the influence of Jiangu decoction(健骨方) on peroxisome proliferator-activated receptor γ(PPARγ)in the femoral head of rabbits with steroid-induced femoral head necrosis.Methods:Eighteen adult SPF healthy New Zealand rabbits were divided into 3 groups:control group,model group,Jiangu decoction(健骨方) group.The rabbits of Jiangu decoction(健骨方) group orally received Jiangu decoction(健骨方) suspension with a dose of 10 ml/kg each day and the drug content was 0.719 g/ml.The rabbits in control and model groups were given saline with a dose of 10 ml/kg.The methylprednisolone sodium succinate was injected intramuscularly into left leg with a dose of 40 mg/kg.Then the rabbits were fed continuously for 3 weeks.The glucocorticoid levels,PPARγ and plasma glucocorticoid levels in the femoral head were measured before and after modeling.Results:Before model established,the plasma glucocorticoid levels had no significant difference among three groups(P=0.301).At 3 weeks after model established,the plasma glucocorticoid level of rabbits in model group increased compared to the control group(P=0.001);and the plasma glucocorticoid level of rabbits in Jiangu decoction(健骨方)group decreased compared with model group(P=0.001).The glucocorticoid level in the local femoral head of rabbits in model group increased compared to the control group(P=0.001);and the glucocorticoid level in the local femoral head of rabbits in Jiangu decoction(健骨方) group decreased compared with model group(P=0.001).The PPARγ level in the local femoral head of rabbits in model group increased compared to the control group(P=0.018);and the PPARγ level in the local femoral head of rabbits in Jiangu decoction(健骨方) group decreased compared with model group(P=0.033).Conclusion:The Jiangu decoction(健骨方) is effective to inhibit the femoral head adipogenic differentiation by decrease the PPAR content,so as to prevent and treat steroid-induced femoral head necrosis.