CLONING AND CHROMOSOME LOCALIZATION OF THE LINKED SCAR MARKER (P1-P2762) GENE IN EARLY WALNUT (DODE)

The objectives of this paper were (1) to clone the closely linked SCAR markers (P1-P2762) gene of the Xinjiang early walnut, and (2) in situ hybridization analysis of the location of the gene on the chromosome. Shoot tips of Xinjiang early walnut were used (1) to clone full-length cDNA sequences of linked markers SCAR (P1-P2762) gene by RT-PCR and RACE and (2) to conduct chromosome sectioning. In situ hybridization occurred when the BCIP/NBT color liquid was diluted with 0.01 M TBS at 1 : 20. The full length gene is 1401 bp, with 320 bp containing a maximum of ORF, encoding 106 amino acids. Nucleic acid homology to the gene shows that the homology with the walnut microsatellite (EF408815.1) sequence was 84%, other. The precocious Walnut SCAR marker (P1-P2762) gene is closely linked with the precocious, but does not directly control the fruit in it’s early stages of development.