Purification of Strep(II)-tagged proteins using new prepacked columns

The Strep(II)-tag is ideal as an affinity tag due to the small size (8 aa) often making removal of the tag unnecessary. The new StrepTrapTM HP 1 ml and 5 ml columns have been specially developed for high specificity towards the Strep(II)-tag and the prepacked format facilitate fast and convenient purification. Purification is done under physiological conditions and mild elution preserves the activity of the target protein. The small bead size of the medium (34 μm) allows elution in narrow peaks minimizing the need for further concentration steps.