A direct real-time polymerase chain reaction assay for rapid high-throughput detection of highly pathogenic North American porcine reproductive and respiratory syndrome virus in China without RNA purification

Kang Kang,Keli Yang,Jiasheng Zhong,Yongxiang Tian,Limin Zhang,Jianxin Zhai,Li-Li Zhang,Changxu Song,Christine Yuan Gou,Jun Luo,Deming Gou

A direct real-time polymerase chain reaction assay for rapid high-throughput detection of highly pathogenic North American porcine reproductive and respiratory syndrome virus in China without RNA purification

2014

Kang Kang
Keli Yang
Jiasheng Zhong
Yongxiang Tian
Limin Zhang
Jianxin Zhai
Li-Li Zhang
Changxu Song
Christine Yuan Gou
Jun Luo
Deming Gou

Background Porcine reproductive and respiratory syndrome virus (PRRSV), and particularly its highly pathogenic genotype (HP-PRRSV), have caused massive economic losses to the global swine industry.

Keywords:

REAL-TIME POLYMERASE CHAIN REACTION ASSAY
Porcine reproductive and respiratory syndrome virus
Virology
Genetics
Genotype
Biology
RNA extraction
highly pathogenic
Detection limit
Reverse transcription polymerase chain reaction
Virus
RNA

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