The Ralstonia solanacearum effector RipAK suppresses plant hypersensitive response by inhibiting the activity of host catalases

Summary The destructive bacterial pathogen Ralstonia solanacearum delivers effector proteins via a type III secretion system for its pathogenesis of plant hosts. However, the biochemical functions of most of these effectors remain unclear. RipAK of R. solanacearum GMI1000 is a type III effector with unknown functions. Functional analysis demonstrated that in tobacco leaves, ripAK knockout bacteria produced an obvious hypersensitive response; also, infected tissues accumulated reactive oxygen species in a shorter period post-infection, compared with wild type. This strongly indicates that RipAK can inhibit hypersensitive response during infection. Further analysis showed that RipAK localizes to peroxisomes and interacts with host catalases (CATs) in plant cells. Truncation of two putative domains of RipAK caused it to fail to target the peroxisome and fail to interact with AtCATs, suggesting that RipAK localization depends on its interaction with CATs. Furthermore, heterologous expression of RipAK inhibited CAT activity in vivo and in vitro. Finally, compared with the ripAK mutant, infection with a bacterial strain overexpressing RipAK inhibited the transcription of many immunity-associated genes in infected tobacco leaves at 2 and 4 hour post-infection, while mRNA levels of NtCAT1 were up-regulated. These data indicate that GMI1000 suppresses hypersensitive response by inhibiting host CATs through RipAK at early stages of infection.