Establishment and Characterization of New Cell Line (YMB-1) Derived from Human Breast Carcinoma

A continuous cell line (YMB-1), derived from a primary breast cancer lesion has been established and maintained in culture for the past 9 months. Cell colonies consist of polygonal epithelial cells containing junctional complexes and tubular formation. The cell population doubling time is 44 hr and the saturation density at confluency is 2. 5x105 cells/cm2• The chromosome modal number is 73 and 3 marker chromosomes are visible. Both cytosol estrogen and androgen receptors are present. However, the cytosol progesterone receptor was not detected by the dextran coated chacoal method. The s. c. injection of 5x106 cells from cell culuture into nude mice induced a tumor. Both the spillage method and cell separation by specific gravity were employed to remove fibroblasts. INTRODUCTION MATERIALS AND METHODS