Partitioning of dietary nitrogen in response to feeding cereal grain supplements to dairy cows during four periods of an extended lactation

Holstein–Friesian dairy cows managed in pasture-based systems are capable of lactating for considerably longer than the traditional 300 days. However, the partitioning of dietary nutrients in the second 300 days of an extended lactation has been shown to be different from that in the first 300 days. The partitioning of dietary nitrogen (N) was investigated using two groups of eight Holstein cows in four experimental periods of 6 days, at 110, 270, 450 and 560 days in milk (DIM). During each period, cows were housed in individual stalls where N intake and output were measured. Cows in a control group received a forage-based diet ad libitum, whereas cows in a second group received 5 kg DM of cereal grain and the basal forage diet ad libitum. N intake was positively related to outputs of milk N, urinary N and faecal N, and negatively to N-use efficiency (NUE). DIM was positively related to output of urinary N and negatively related to NUE and outputs of milk N and faecal N. The partitioning of N in the second year of an extended lactation was different from that in the first year, meaning that the partitioning of N reported for cows up to 300 DIM cannot be used to accurately predict the partitioning of N in cows beyond 300 DIM. Adding grain to the diet increased total nitrogen intake but decreased NUE. However, it is plausible for the addition of cereal grain to the diet of dairy cows to increase NUE, but only when the concentration of dietary crude protein decreases and any increase in dry-matter intake is small, such that total nitrogen intake is decreased.