How unsaturated fatty acids and plant stanols affect sterols plasma level and cellular membranes? Review on model studies involving the Langmuir monolayer technique

Abstract The Langmuir monolayer technique has long been known for its usefulness to study the interaction between molecules and mimic cellular membranes to understand the mechanism of action of biologically relevant molecules. In this review we summarize the results that provided insight into the potential mechanism for lowering the plasma level of cholesterol by hypocholesterolemic substances (unsaturated fatty acids (UFAs) and phytocompounds) - in the aspect of prevention of atherosclerosis - and their effects on model biomembranes. The results on UFAs/cholesterol (oxysterols) interactions indicate that these systems are miscible and strongly interacting, contrary to immiscible systems containing saturated fatty acids. Lowering of cholesterol plasma level by UFAs was attributed to the strong affinity between UFAs and sterols, resulting in the formation of high stability complexes, in which sterols were bound and eliminated from the body. Studies on the effect of UFAs and plant sterols/stanols on simplified biomembranes (modeled as cholesterol/DPPC system) indicated that the studied hypocholesterolemic substances modify the biophysical properties of model membrane, affecting its fluidity and interactions between membrane components. Both UFAs and plant sterols/stanols were found to loosen interactions between DPPC and cholesterol and decrease membrane rigidity caused by the excess cholesterol in biomembrane, thus compensating strong condensing effect of cholesterol and restoring proper membrane fluidity, which is of utmost importance for normal cells functioning. The agreement between model - in vitro - studies and biological results prove the usefulness of the Langmuir monolayer technique, which helps in understanding the mode of action of biologically relevant substances.