Understanding the interdependence of strain of electrotroph, cathode potential and initial Cu(II) concentration for simultaneous Cu(II) removal and acetate production in microbial electrosynthesis systems

Abstract Metallurgical microbial electrosynthesis systems (MES) are holding great promise for simultaneous heavy metal removal and acetate production from heavy metal-contaminated and organics-barren waters. How critical parameters of strain of electrotroph, cathode potential and initial heavy metal concentration affect MES performance, however, is not yet fully understood. Heavy metal of Cu(II) and four Cu(II)-tolerant electrotrophs (Stenotrophomonas maltophilia JY1, Citrobacter sp. JY3, Pseudomonas aeruginosa JY5 and Stenotrophomonas sp. JY6) were employed to evaluate MES performance at various cathode potentials (−900 or −600 mV vs. standard hydrogen electrode) and initial Cu(II) concentrations (60–120 mg L−1). Each electrotrophs exhibited incremental Cu(II) removals with increased Cu(II) at −900 mV, higher than at −600 mV or in the abiotic controls. Acetate production by JY1 and JY6 decreased with the increase in initial Cu(II), compared to an initial increase and a decrease thereafter for JY3 and JY5. For each electrotrophs, the biofilms than the planktonic cells released more amounts of extracellular polymeric substances (EPS) with a compositional diversity and stronger Cu(II) complexation at −900 mV. These were higher than at −600 mV, or in the controls either under open circuit conditions or in the absence of Cu(II). This work demonstrates the interdependence of strain of electrotroph, cathode potential and initial Cu(II) on simultaneous Cu(II) removal and acetate production through the release of different amounts of EPS with diverse composites, contributing to enhancing the controlled MES for efficient recovery of value-added products from Cu(II)-contaminated and organics-barren waters.