CORRECTION OF DISORDERS OF THE REPRODUCTIVE FUNCTION OF MALE RATS USING GADOLINIUM ORTHOVANADATE NANOPARTICLES

Male rats were exposed to emotional stress and had an excessive amount of phytoestrogens with the mother’s milk (from 3 to 22 days of life) and were examined at an age of 10 months. Animals had an excess body weight through an increased weight of visceral fat, changes in protein and lipid metabolism (increased total cholesterol and triglycerides), impaired pro/antioxidant balance. These males had a decreased level of testosterone and impaired spermatogenesis (less total sperm concentration and less concentration of morphologically normal gametes) with the formation of defective spermatozoa. Also, the violations of the sexual behavior and the worst fertilization of females were found. More than a two-fold increase of intrauterine losses in these pregnant females was noted. Due to such changes, the reproductive potential (Fi ) in these males was reduced by half compared to the control level. The effectiveness of application of gadolinium orthovanadate nanoparticles activated by europium (NPs GdVO4 :Eu3+), as part of a hydrogel or a pharmacological composition (0.33 mg/kg of body mass, 70 days) for correction of male reproductive function disorders was studied. As reference preparations, tribestane (68 mg/kg) or spemane (168 mg/kg) was used. After the treatment, the results of spermatogenesis, testosterone production and fertility of the main group of males became better. Due to the reduction of embryonic losses, the reproductive potential index was normalized. NPs normalized the level of triglycerides, total cholesterol, arginine in the serum, as well as the transaminase activity in the liver. An increase in the weight and gonadotropic activity of hypophysis of the treated males were found, indicating a possible effect of NPs on the central regulatory mechanism of the reproductive function. The higher biological activity of NPs in the hydrosol composition was found, which can be explained by the introduction of auxiliary substances into the pharmacological composition that affects the physicochemical properties of NPs GdVO4 :Eu3+ and their biological activity.