Isolation and identification of a bacterial strain JS018 capable of degrading several kinds of organophosphate pesticides

Organophosphate pesticides are used widely all over the world and play an important role in plant pest control.However these pesticides are considered as pollutants and harmful to human health.To search for microorganisms that can degrade organophosphate pesticides with high efficiency,a bacterial strain,coded as JS018,was isolated and screened from the soil in the vicinity of Shanming Pesticides Factory,Shanming,Fujian.Laboratory tests showed that the bacterium could degrade several kinds of organophosphate pesticides,such as Parathion-methyl and phoxin.The strain's degrading rates on phoxin,Parathion-methyl,hostathion and dichlorvos in LB liquid fermentation medium for 36 h were 99%,96%,80.4%and 69.0% respectively.The bacterial colonies on LB plate appeared shiny and pale-pink in color.The bacteria were Gram-negative coccoids,0.5～0.7μm in diameter.They grew well at 30～38℃ and pH7.0～9.0.The optimal temperature and pH for cell growth was 32℃ and pH 7.5～8.0,respectively.They did not grow in medium containing 6% or more NaCl.The antibiotic susceptibility tests showed that the strain was resistant to ampicillin,penicillin and lincomycin.It was sensitive to kanamycin,tetracycline and gentamicin.Laboratory tests also showed that the strain could ferment D-glucose,trehalose,melezitose and ethanol.It was negative in the production of indole and hydrogen sulfide.It could not liquefy gelatin,utilize citrate,nor ferment L-arabinose,sucrose,D-mannitol,D-xylose,fructose,D-galactose,maltose or lactose.The catalase,urease and nitrate reduction were positive.Based on its morphological,physiological and biochemical properties as well as the 16S rDNA sequence analysis result,the strain was tentatively identified as Roseomonas sp.