Anti-inflammation Activities of Cultured Products from Suspension Culture of Aloe vera Callus

Abstract Cultured products (callus and exopolysaccharide)were obtained from suspension culture of Aloe vera callus, and theextracts of callus were further prepared with cold water or 60%ethanol solution. The ethanol extract of callus (AC) andexopolysaccharide (ACP) of 10 mg/mL exhibited the relativelyhigher suppression activity of 43.2-52.1% against hyaluronidaseactivity. Thus, their anti-inflammatory effects were furtherinvestigated using animal cell (Raw 264.7) in vitro. Though ACshows a slight suppression effect of cell survival rate (97%) usingMTT assay in the presence of 400µg/mL AC- dimethyl sulfoxide(DMSO), cell growth promotion was observed in the othersamples of lower levels. It indicates that the ethanol extract ofAloe callus rarely affect cell survival rate in the ranges (200−400µg/mL) used in the study. Using Griess reagent, the suppression ofNO production by the aloe callus extract was analyzed bymeasuring the amount of the nitrite produced in Raw 264.7culture activated by lipopolysaccharide (LPS). As a result,supplementation of AC-distilled water (DW) and AC-DMSOproduced higher levels of NO than the positive control LPS.However, the NO suppression effect by ACP-DW was so intensethat lower amount (80−100 µg/mL) suppressed NO production tothe level of the control. The effect was attributed to the expressionof the iNOS. Then, Raw 264.7 cells were stimulated with the LPSand expression of COX-2 protein level was analyzed dependingon the Aloe suspension culture product treatment. The resultsshowed that the ACP-DW supplemented medium did not expressCOX-2 by itself, and LPS stimulated COX-2 expression wasslightly decreased. On the other hand, realtime-PCR analysis ofthe expression of inflammatory cytokine showed that IL-1β andTNF-α expression was highly suppressed in the ACP- distilledwater supplemented medium.Keywords Aloe vera callus · anti-inflammation activity · cultured products · extracts · suspension culture서론Aloe vera 잎은 4다당류(Mw: 10−10