Comparative scrutinize of BSA and HEWL in the vicinity of metallo-catanionic aggregates derived from single chain metallosurfactant and anionic surfactant
2022
Abstract In recent times, protein-surfactant interactions have been extensively explored due to their stimulating applications in different fields. These systems have efficaciously addressed the economic, environmental, and ecological issues. Sensing their prominence, through this report, we investigate and compare the interactional behavior of Bovine Serum Albumin (BSA) and Hen Egg White Lysozyme (HEWL) with metallo-catanionic aggregate mixtures. Attempts have been made to synthesize the metallo-catanionic aggregates from single-chain metallosurfactant (FeCTAC I) and anionic surfactant (SDS) i.e., FeCTAC I:SDS. Before proceeding towards the interactional studies of metallo-catanionic aggregates with model proteins, the morphology of formed aggregates was elucidated. Here, out of different possible molar ratios of FeCTAC I:SDS, two ratios i.e 70:30 and 30:70 were opted. The aggregates were examined through conductivity, Dynamic Light Scattering (DLS), Field Emission Scanning Electron Microscopy (FESEM), Confocal Laser Scanning Microscopy (CLSM), Small Angle X-Ray Scattering (SAXS), Zeta Potential, and Vibrating Sample Magnetometer (VSM) techniques. Interestingly, the SAXS analysis demonstrated the micellar behavior of 70:30 and vesicular behavior of 30:70 aggregates mixture, due to the difference of overall charge on them. The interactional study of BSA and HEWL with FeCTAC I:SDS was carried out through SDS-PAGE, absorption, emission, and circular dichroism (CD) spectroscopy. The effect of FeCTAC I:SDS on the linear polypeptide chain of BSA and HEWL was observed to be different, however, the secondary and tertiary structures were affected similarly. To elucidate the exact locality of FeCTAC I:SDS aggregates on the BSA, a site marker experiment was carried out. The pyrene extrinsic fluorescence experiment was performed to verify the type of interactions among HEWL and metallo-catanionic aggregates. From, the comparative studies, we concluded that the overall system was controlled by the type of forces playing between the protein and aggregate mixture that emerged from the total charge on the protein as well as on the aggregates. These types of metallo-catanionic aggregates that are flexible to shape are considered as the new alternatives for nano-vehicle applications.
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