Ginsenoside Rbl reduces intestinal ischemia-reperfusion induced acute lung injury in mice：role of nuclear factor erythroid 2.related factor 2/heme oxygenase-1 signaling

Objective To investigate the effectsof ginsenosi-de Rbl on acute lung injury induced by intestinal ischemia reperfusion in mice and role of nuclear factor erythroid 2-related factor 2 （Nri2） / heme oxygenase-1 （HO-1） pathway in the pathophysiologic process. Methods Male C57BL/6J mice were randomly divided into 5 groups ： （ 1 ） Sham group （ S group） ; （ 2 ） Intestinal Ischemia Reperfusion group （I/R group） ; （ 3 ） Intestinal Isehemia Reperfusion ＋ 60 mg/kg ginsenoside Rbl （ I/R ＋ Rb 1 group） ; （4） all-trans retinoic acid （ATRA） ＋ I/R group （ ATRA ＋ I/R group） ; （5） ATRA ＋ I/R ＋ 60 mg/kg ginseuoside Rbl （ ATRA ＋ I/R ＋ Rbl group）. The Western blotting analysis were performed to ob- serve the expression of Nrf2 and HO-1 ; tumor necrosis factor-α （TNF-ct）, interleukin （IL）-6, IL-10, su- peroxide dismutase （SOD） and malondialdehyde （MDA） were measured;Lung histology was observed and evaluated. Results Intestinal ischemia reperfusion can increase lung injury which was characterized by Nrf2, HO-1, TNF-α, IL-6, MDA, edema （P 〈 0. 05）. Rbl can reduce the Intestinal ischemia reperfusion which decreased by Nrl2, HO-1, TNF-α, IL-6, MDA, edema （P 〈 0. 05）. After administration of ATRA, which was an antagonists of Nrf2, Nrf2, HO-I, TNF-α, IL-6, MDA, edema were increased （ P 〈 0. 05 ）. SOD was opposite as well as IL-10. Conclusion Ginsenoside Rbl attenuates acute lung injury induced by intestinal ischemia reperfusion by activating Nrf2/HO-1 pathway. Key words: Nuclear factor erythroid 2-related factor 2 ;  Heme oxygenase-1 ;  Ginsenoside Rbl ; Acute lung injury;  Intestinal ischemia/reperfusion injury