SSR Analysis of Maize Regenerated Haploid Plants

10 haploid regenerated maize(Zea mays L.) plants were analyzed using 47 pairs of SSR markers.Results showed that no somaclonal variation was found in the haploid plants as their banding patterns were all from their parents'.Among the 47 SSR markers,29 were polymorphic between maize inbred line Zheng58 and B73;while 16 were polymorphic between Xi502 and Chang72.According to the analysis on genetic similarity coefficient,the 6 haploid plants derived coming from Zheng58/B73 were genetic segregated toward B73 as their genetic similarity coefficients with Zheng58(51.06%～61.70%) were all smaller than that with B73(76.60%～87.23%);While no segregation distortion was detected in the 4 haploid plants with genetic background of Xi502/Chang72.